During the game, one card from the deck is drawn at a time, and anyone whose board includes that card's picture marks it on their board. A player wins by marking four pictures that form one of four patterns, as exemplified below: any entire row, any entire column, the four corners of the grid and any 2-by-2 square.

Loteria has been played as a game of chance, as a pastime, and for educational purposes. Because the Loteria cards include the name of the pictured character, they are used to teach reading, writing, history, and social values.